Serum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. The most common indications for a serum protein electrophoresis test are to diagnose or monitor multiple myeloma, a monoclonal gammopathy of uncertain significance (MGUS), or further investigate a discrepancy between a . Evaluation reveals ONE restricted band(s) migrating in the BETA-2 region(s), respectively. Estimated concentration(s): 0.5 g/dL, respectively. Consider immunofixation analysis if indicated and not already ordered.. Serum protein electrophoresis (SPEP) is an inexpensive, easy-to-perform screening procedure for initial identification of monoclonal bands. Monoclonal bands are usually seen in the gamma zone, but may be seen in proximity to the beta band or rarely in the alpha-2 region. Blood can be collected in a tube with a clot activator, and after separation from blood components, serum is then placed on special paper treated with agarose gel followed by exposure to an electric current in the presence . Answers from doctors on monoclonal band in gamma region. First: A monclonal band suggests the abnormal production of antibody by a single expanded clone of a b-cell lymphocyte. Sometimes this is a malignancy issue (myeloma) some times it is not (mgus). In either case, this should be monitored by a hematology-oncology physician.. An M-protein usually presents as a single narrow peak, resembling a "church spire," in the gamma, beta, or alpha-2 region of the densitometer tracing, or as a dense, discrete band on the agarose gel (see image below).. Among the 16 M band positive cases that we studied, there was an M spike in the gamma region in 14 (87.5%) cases and 2 cases(12.5%) had an M band in the beta region. Col.G S Chopra et al reported that, 84.8% of the cases had an M band in the gamma (γ) region and that 15.2% cases had an M spike in the beta (β) globin region ..